Promoting bioremediation of brewery wastewater, production of bioelectricity and microbial community shift by sludge microbial fuel cells using biochar as anode.

Seeking low-cost and eco-friendly electrode catalyst of microbial fuel cell (MFC) reactor has received extensive attention in recent decades. In this study, a sludge MFC was coupled with biochar-modified-anode (BC-300, BC-400, and BC-500) for actual brewery wastewater treatment. The physicochemical properties of biochar largely depended on the pyrolysis temperature, further affecting the removal efficiency of wastewater indicators. BC-400 MFC proved to be efficient for TN and NH4+-N removal, while the maximum removal efficiencies of COD and TP were achieved by BC-500 MFC, reaching respectively 97.14 % and 89.67 %. Biochar could promote the degradation of dissolved organic matter (DOM) in wastewater by increasing the electrochemical performances of MFC. The maximum output voltage of BC-400 MFC reached 410.24 mV, and the maximum electricity generation of 108.05 mW/m2 was also obtained, surpassing the pristine MFC (BCC-MFC) by 4.67 times. High-throughput sequencing results illustrated that the enrichment of electrochemically active bacteria (EAB) and functional bacteria (Longilinea, Denitratisoma, and Pseudomonas) in BC-MFCs, contributed to pollutants degradation and electron transfer. Furthermore, biochar affected directly the electrical conductivity of wastewater, simultaneously changing microbial community composition of MFC anode. Considering both enhanced removal efficiency of pollutants and increased power generation, the results of this study would offer technical reference for the application of biochar as MFC catalyst for brewery wastewater treatment.

Ambient Air Pollution and Vision Disorder: A Systematic Review and Meta-Analysis.

The effects of air pollution on physical health are well recognized, with many studies revealing air pollution's effects on vision disorder, yet no relationship has been established. Therefore, a meta-analysis was carried out in this study to investigate the connection between vision disorder and ambient particles (diameter ≤ 2.5 µm (PM2.5), diameter ≤ 10 µm (PM10)) and gaseous pollutants (nitrogen dioxide (NO2), sulfur dioxide (SO2), carbon monoxide (CO), Ozone (O3)). Twelve relevant studies published by 26 February 2024 were identified in three databases. A pooled odds ratios (ORs) of 95% confidence intervals (CIs) were obtained using random-effects meta-analysis models. Meta-analysis results revealed that for every 10 µg/m3 increase in PM2.5 and NO2 exposure, a substantially higher incidence of vision disorder was observed (OR = 1.10; 95% CI: 1.01, 1.19; OR = 1.08, 95% CI: 1.00, 1.16). No significant correlation existed between exposure to PM10, SO2 and CO and vision disorder. However, O3 exposure was negatively associated with vision disorder. In addition, subgroup analyses revealed that PM2.5 exposure was significantly correlated with the risk of glaucoma and age-related macular degeneration and that children and adolescents were more susceptible to NO2 and PM2.5 than adults. Overall, exposure to air pollutants, especially PM2.5 and NO2, may increase the incidence of vision disorder.

Improving bioelectrochemical performance by sulfur-doped titanium dioxide cooperated with Zirconium based metal-organic framework (S-TiO2@MOF-808) as cathode in microbial fuel cells.

The sulfur-doped titanium dioxide (S-TiO2) cooperated with Zirconium based on a kind of metal-organic framework (MOF-808) was successfully prepared as cathode catalyst (S-TiO2@MOF-808) of microbial fuel cell (MFC) by two-step hydrothermal reaction. The particle size was approximately 5 µm, and the spherical S-TiO2 particle was attached to the surface of MOF-808 as irregular block solid. Zr-O, C-O and O-H bond were indicated to exist in S-TiO2@MOF-808. When n (Zr4+): n(Ti4+) was 1: 5, S-TiO2@MOF-808 showed better oxygen reduction reaction (ORR). The introduction of S-TiO2 restrained the framework collapse of MOF-808, S-TiO2@MOF-808 showed much higher catalytic stability in reaction. The recombination of sulfur and TiO2 reduced the charge transfer resistance, accelerated the electron transfer rate, and improved ORR greatly. The maximum power density of S-TiO2@MOF-808-MFC was 84.05 mW/m2, about 2.17 times of S-TiO2-MFC (38.64 mW/m2). The maximum voltage of S-TiO2@MOF-808-MFC was 205 mV, and the stability was maintained for 6 d.

Research status and prospect of nano silver (Ag)-modified photocatalytic materials for degradation of organic pollutants.

Nano silver (Ag) was metallic Ag monomers with particle size to the nanoscale. Photocatalyst was a kind of semiconductor material with photocatalytic function. Loading precious metal Ag onto semiconductor surfaces by microwave, laser-induced, solvent-thermal and hydrothermal methods could capture photogenerated electrons, reduced the compounding rate of holes and photogenerated electrons during the photocatalytic process, thereby improving the electron transfer efficiency of photocatalysis and enhancing the absorption of visible light by silver nanoparticles through the plasma resonance effect. The highly reactive free radicals produced by photocatalysts were used in the organic degradation process to degrade organic matter into inorganic matter and was a faster, more efficient and less polluting method of pollutant degradation, which has attracted a lot of attention from researchers. This review discussed the modification of various types of photocatalysts by nano Ag through different methods. The photocatalytic degradation of dyes, antibiotics and persistent organic pollutants by different modified composites was also analyzed. This review covered the several ways and means in which nano Ag has modified diverse photocatalytic materials as well as the photocatalytic degradation of dyes, antibiotics and persistent organic pollutants. This review identified the drawbacks of the existing nano Ag-modified photocatalytic materials, including their low yield and lack of recyclability, and it also offered suggestions for potential future directions for their improvement. The purpose of this review was to further research on the technology of nano Ag-modified photocatalytic materials and to encourage the creation of new modified photocatalytic nanomaterials for the treatment of organic pollutant degradation.

[Remediation of Three Oxidants on Polycyclic Aromatic Hydrocarbons in Coking Contaminated Soil and Its Response to Indigenous Microorganisms].

To explore the influences of chemical oxidation on the physiological and ecological functions of indigenous microorganisms during contaminated soil remediation, three oxidants, including KMnO4, Na2S2O8, and O3, were selected to investigate their remediation effects on PAHs and the responses to indigenous microorganisms under different liquid-solid ratios, in this study. The results showed that:when the ΣPAHs concentration was 679.1 mg·kg-1 and the dosage of KMnO4 and Na2S2O8 was 1%, the removal efficiency of ΣPAHs reached up to 96.9% and 95.7% under the liquid-solid ratio of 6:1; for the O3 treatment, the removal efficiency of ΣPAHs was the highest(82.3%) at the O3 dosage and the liquid-solid ratio of 72 mg·min-1 and 8:1, respectively. The removal efficiency of low ring(3-4 rings) PAHs was higher than that of high ring(5-6 rings) PAHs under different liquid-solid ratios. The highest removal efficiencies were observed for phenanthrene and acenaphthene, whereas for benzo[a]pyrene, only the KMnO4treatment provided an effective performance, showing the highest removal efficiency of 97.4%. The microbial quantity analysis indicated that the quantity of soil microorganisms in the soil dropped sharply after being treated with KMnO4, decreasing from 108 copies·g-1 to 105 copies·g-1, whereas it changed only slightly after being treated with Na2S2O8 and O3. The community structure analysis showed that Proteobacteria were predominant in the contaminated soil, with the relative abundance of 99.5%. The addition of KMnO4 and Na2S2O8 significantly increased the microbial diversity; in particular, the relative abundance of a variety of microorganisms(such as Ralstonia and Acinetobacter) that can degrade PAHs was remarkably increased. The analysis of microbial metabolic function pathways revealed that chemical oxidation could simultaneously increase the relative abundance of PAHs-degrading bacteria and improve the ability of organic metabolism. Overall, the KMnO4 treatment greatly altered the quantity of microorganisms and the structure of the microbial community and the relative abundance of PAHs-degrading microorganisms at the liquid-solid ratio of 6:1.

Adsorption of hexavalent chromium, Rhodamine B and Congo red simultaneously in aquatic by zeolitic imidazolate framework coupling carbon nanotubes.

Zeolitic imidazolate framework/carbon nanotube (ZIF-67/CNTs) was prepared by precipitation method. ZIF-67/CNTs maintained the characteristics of large specific surface area and high porosity of ZIFs, showing stable cubic structure. The adsorption capacities of ZIF-67/CNTs for Cong red (CR), Rhodamine B (RhB) and Cr(VI) were 36.82 mg/g, 1421.29 mg/g and 716.67 mg/g under the conditions of 2:1, 3:1 and 1:3 masses of ZIF-67 and CNTs, respectively. The optimum adsorption temperature of CR, RhB and Cr(VI) were 30 °C, and the removal rates at the adsorption equilibrium were 81.22%, 72.87% and 48.35%. The adsorption kinetic model of the three adsorbents on ZIF-67/CNTs was consistent with the quasi-second order reaction model, and the adsorption isotherms were more consistent with adsorption law of Langmuir. The adsorption mechanism for Cr(VI) was mainly electrostatic interaction, and the adsorption mechanism for azo dyes was the combination of physical and chemical adsorption. This study would provide theoretical basis for further developing metal organic framework (MOF) materials for environmental applications.

Trace N-doped manganese dioxide cooperated with Ping-pong chrysanthemum-like NiAl-layered double hydroxide on cathode for improving bioelectrochemical performance of microbial fuel cell.

Trace N-doped manganese dioxide (MnO2) nanoparticles were attached to NiAl-layered double hydroxide (LDH) nano sheets by a simple two-step hydrothermal reaction, and N-MnO2@NiAl-LDH was successfully prepared as cathode catalyst of microbial fuel cell (MFC). N-MnO2@NiAl-LDH was Ping-pong chrysanthemum-like structure formed by overlapping lamellar structures, with spherical MnO2 particles attached on. The unique Ping-pong chrysanthemum-like structure and pore size distribution provided large number of electrochemical active sites. The recombination of trace N and MnO2 reduced the charge transfer resistance, accelerated the electron transfer rate, and N-MnO2@NiAl-LDH showed high oxygen reduction reaction (ORR) capability. The maximum output power density of N-MnO2@NiAl-LDH-MFC was 698 mW/m2, about 4.59 times of NiAl-LDH (152.1 mW/m2). The maximum voltage was about 320 mV, and the stability was good for about 7 d. This would provide technical reference for the utilization of cathode catalyst for fuel cells.

Improved bioelectrochemical performance of MnO2 nanorods modified cathode in microbial fuel cell.

The property of cathode in the microbial fuel cell (MFC) was one of the key factors limiting its output performance. MnO2 nanorods were prepared by a simple hydrothermal method as cathode catalysts for MFCs. There were a number of typical characteristic crystal planes of MnO2 nanorods like (110), (310), (121), and (501). Additionally, there were great many hydroxyl groups on the surface of nanorod-like MnO2, which provided a rich set of active adsorption sites. The maximum power density (Pmax) of MnO2-MFC was 180 mW/m2, which was 1.51 times that of hydrothermally synthesized MnO2 (119.07 mW/m2), 4.28 times that of naturally synthesized MnO2 (42.05 mW/m2), and 5.61 times that of the bare cathode (32.11 mW/m2). The maximum voltage was 234 mV and the maximum stabilization time was 4 days. The characteristics of MnO2, including rod-like structure, high specific surface area, and high conductivity, were conducive to providing more active sites for oxygen reduction reaction (ORR). Therefore, the air cathode modified by MnO2 nanorods was a kind of fuel cell electrode with great application potential.

A pathway for promoting bioelectrochemical performance of microbial fuel cell by synthesizing graphite carbon nitride doped on single atom catalyst copper as cathode catalyst.

In this study, a simple distributed feeding method was used to dope graphite phase carbon nitride (g-C3N4) on single atom catalyst (SAC) copper (Cu) to form composite material (Cu-SA/CN). Cu-SA/CN was formed by mutual doping of polyhedral block Cu and irregular g-C3N4. There were obvious crystal face peaks at 28.4, 43.3, 47.3 and 56.2°. Large solid Cu and small irregular g-C3N4 were successfully combined and C, Cu, N and O elements were uniformly distributed on the surface of Cu-SA/CN. The valence bond of N-CN, C-NC, CC and OH was found. When the Cu content was 0.03 mol, Cu-SA/CN3 showed excellent redox activity. The maximum power density of Cu-SA/CN3-MFC was 456.976 mW/m2, the maximum voltage was 599 mV, which could be stable for 7 d. Cu-SA/CN3 was proved to provide more electrically active sites, strong catalytic oxygen reduction ability and conductivity.

Performance and microbial mechanism of eletrotrophic bio-cathode denitrification under low temperature.

Insufficient amount of carbon in wastewater and low temperatures hinder the use of biological nitrogen removal for purification of wastewaters. Nitrogen removal using cold-tolerant electrotrophic cathodic microbes is a novel and unique autotrophic denitrification technique in which electrical current, not chemicals, is used as a source of electrons. In this study, integrated MFC (RW) and open-circuit MFC (RO) were cultured and acclimatized in stages at a low temperature (10 °C) to impart cold tolerance to electrotrophic cathodic microbes, investigate the effectiveness of simultaneous nitrification and denitrification (SND) process, and address the possible mechanism of microbial action. The results showed that (i) microbial communities in the RW system were successfully enriched with the cold-tolerant electrotrophic cathodic microbes after five stages, and (ii) the degree of NH4+-N removal and SND were 75.50% and 81.91%, respectively, but the respective values in the RO system were only 40.47% and 54.01%. The desirable SND efficiency was obtained in RW at a DO of ∼0.6 mg/L, a current of ∼20 mA, and pH ∼7.0. In RW, Thauera, Pesudomonas, and Hydrogenophaga were the main electrotrophic cathodic denitrifying bacteria with cold tolerance capable of degrading ammonia, nitrate, and nitrite through autotrophic denitrification and cathodic-driven bio-electrochemical denitrification. Besides, for RW, results from high throughput sequencing analysis revealed that the abundance of genes related to energy production and conversion, amino acid transport, and metabolism, signal transduction, environmental adaptation, and enzymatic activity (AMO, HAO, NAR, NIR, NOR, and NOS) were significantly higher than the corresponding parameters of the RO system. This may explain the reason behind RW having excellent ammonia and TN removal performance at low temperatures.

A critical review of research progress for metal alloy materials in hydrogen evolution and oxygen evolution reaction.

Hydrogen produced by electrolyzing water has attracted extensive attention as an effective way to generate and store new energy by using renewable energy. Electrocatalytic hydrogen evolution reaction (HER) and oxygen evolution reaction (OER) were the core reactions in the process of hydrogen production by water electrolysis, however, due to the low efficiency of the electrolytic device caused by its slow kinetic reaction and the dependence on noble metal catalysts (platinum and iridium/ruthenium (oxide)), which limited its wide application. The preparation of high-efficiency catalysts with high catalytic activity, stability, low cost and scalability played a vital role in promoting the development of hydrogen production technology from electrolytic water and has become a current research hotspot. Metal alloy catalysts have been widely studied as high-efficiency electrocatalysts. This study introduced and analyzed the mechanism and application of metal alloy catalyst in hydrogen and oxygen evolution reaction, summarized and discussed the progress in the design, preparation and application of metal alloy electrocatalysts. Finally, the strategy and prospect of new high-efficiency electrocatalysts were proposed.

Review on effects of some insecticides on honey bee health.

Insecticides, one of the main agrochemicals, are useful for controlling pests; however, the indiscriminate use of insecticides has led to negative effects on nontarget insects, especially honey bees, which are essential for pollination services. Different classes of insecticides, such as neonicotinoids, pyrethroids, chlorantraniliprole, spinosad, flupyradifurone and sulfoxaflor, not only negatively affect honey bee growth and development but also decrease their foraging activity and pollination services by influencing their olfactory sensation, memory, navigation back to the nest, flight ability, and dance circuits. Honey bees resist the harmful effects of insecticides by coordinating the expression of genes related to immunity, metabolism, and detoxification pathways. To our knowledge, more research has been conducted on the effects of neonicotinoids on honey bee health than those of other insecticides. In this review, we summarize the current knowledge regarding the effects of some insecticides, especially neonicotinoids, on honey bee health. Possible strategies to increase the positive impacts of insecticides on agriculture and reduce their negative effects on honey bees are also discussed.

The avian gut microbiota: Diversity, influencing factors, and future directions.

The gut microbiota is viewed as the "second genome" of animals, sharing intricate relationships with their respective hosts. Because the gut microbial community and its diversity are affected by many intrinsic and extrinsic factors, studying intestinal microbes has become an important research topic. However, publications are dominated by studies on domestic or captive birds, while research on the composition and response mechanism of environmental changes in the gut microbiota of wild birds remains scarce. Therefore, it is important to understand the co-evolution of host and intestinal bacteria under natural conditions to elucidate the diversity, maintenance mechanisms, and functions of gut microbes in wild birds. Here, the existing knowledge of gut microbiota in captive and wild birds is summarized, along with previous studies on the composition and function, research methods employed, and factors influencing the avian gut microbial communities. Furthermore, research hotspots and directions were also discussed to identify the dynamics of the avian gut microbiota, aiming to contribute to studies of avian microbiology in the future.

Enhancing bioelectrochemical performance of two-dimensional material attached by covalent/metal organic frameworks as cathode catalyst for microbial fuel cells.

In this study, covalent/metal organic framework and two-dimensional material (COF-300/ZIF-8@Ti3AlC2) were composited by a three-step distributed feed method for cathode catalyst in microbial fuel cells (MFCs). The growth of irregular cube-like COF-300/ZIF-8 on the Ti3AlC2 substrate was observed. Al, Zn, Ti, N, C and O elements were observed uniformly and more active sites were offered through it. The external output voltage of COF-300/ZIF-8@Ti3AlC2-MFC was 576 mV and this could be almost unchanged for 9 days. The external output power density was 587.01 mW/m2, and that was 1.25 times of COF-300@Ti3AlC2-MFC (469.30 mW/m2) and 1.67 times of COF-300/ZIF-8-MFC (352.09 mW/m2). Ti3AlC2 enhanced the electrical conductivity of the composite by its rich surface functional groups and much more surface active sites. COF-300/ZIF-8 mixture improved the instability and disorder of the monomer. This study would supply technical support for the expanded request of composite materials in microbial electrochemistry.

Influence of heavy metals on Saunders's Gull (Saundersilarus saundersi) reproduction in the Yellow River Estuary: risk assessment and bioaccumulation.

The heavy metal migration in the food chain exerted significant influence on the survival and reproduction of wetland birds and then disturbed and threatened the balance and health of the estuary ecosystem. In this study, the concentration of heavy metals (Cu, Cr, Fe, Mn, Cd, Ni, and Pb) in surface sediment of the Yellow River Estuary (YRE), the food sources of Saunders's Gull (Saundersilarus saundersi) nestlings, and the egg structure of birds were analyzed to determine the bioaccumulation and reproductive influence on wetland bird. The results indicated higher mean concentrations of sediment heavy metals than their corresponding background values in 2019, with the exception of Fe. Notably, the metal Cd exceeded geochemical background value by 1561.5% in 2018 and 1353.9% in 2019, resulting in severe contamination associated with Cd in the YRE (with geo-accumulation indexes of 3.44 and 3.23). Biomagnification factor (BMF) of heavy metals demonstrated that the concentrations of Cr, Ni, and Cu decreased with the trophic level rising while Cd, Mn, Pb, and Fe denoted bio-amplification in the food chain. The residual indexes showed that the food resources of Saunders's Gull were polluted by Cr, Pb, and Cu. Additionally, a higher enrichment of heavy metals was observed in the eggshell membrane. Metal concentrations had significant influences on the reproduction of Saunders's Gull, except for Cd, among which Ni, Pb, Cu, and Fe may have contributed to the reproductive success of birds, whereas the hatching failure of birds may be caused by Cr and Mn. It is of great importance to monitor the contamination of the wetland ecosystem and provide effective management and protection of the wildlife in the YRE.

Historical development and prospect of intimately coupling photocatalysis and biological technology for pollutant treatment in sewage: A review.

Through the synergistic effect of photocatalysis and biodegradation, intimately coupling photocatalysis and biological (ICPB) technology could improve the removal rate and mineralization rate of refractory pollutants and reduce the toxicity of intermediate products. ICPB system was characterized with the advantages of simple operation, low energy consumption and high treatment efficiency. As a new sewage treatment technology, ICPB system has shown great potential in the treatment of refractory pollutants, and has been widely concerned. In this study, the research progress of photocatalyst, carrier and biofilm in ICPB system were discussed, and the degradation mechanism was introduced. The shortcomings of the current ICPB system were pointed out, and the possible research directions of ICPB in the future were proposed. This review aimed to deepen the understanding of ICPB technology and promoted the further development of ICPB technology in the treatment of refractory pollutants.

Enhanced bioelectrochemical performance of microbial fuel cell with titanium dioxide-attached dual metal organic frameworks grown on zinc aluminum - layered double hydroxide as cathode catalyst.

In this study, a three-step distributed feeding method was used to prepare TiO2-attached dual CoZn-metal organic frameworks growing on ZnAl-layered double hydroxide (TiO2@ZIF-67/ZIF-8@ZnAl-LDH) as cathode catalyst of microbial fuel cell (MFC). The composite material was a composite core-shell structure constructed by multi-layer coating with sheet-like ZnAl-LDH as the base, dual MOFs as the magnetic core and TiO2 as the rough surface. The composite material had crystal planes (009), (110), (101) interface. The rough surface, core-shell core and polyhedral structure of TiO2@ZIF-67/ZIF-8@ZnAl-LDH were observed. The complete distribution of Ti, Zn, Al, and Co in the material was observed and offered active sites. The contents of Ti (15.97 %), Al (5.53 %), Na (5.04 %), N (3.52%), Zn (1.47 %) were found out. TiO2@ZIF-67/ZIF-8@ZnAl-LDH was excellent in electrochemical activity and the maximum power density was 409.6 mW/m2, the stable continuous output voltage was 538.4 mV for 8 d.

Rapid degradation of Congo red wastewater by Rhodopseudomonas palustris intimately coupled carbon nanotube - Silver modified titanium dioxide photocatalytic composite with sodium alginate.

With a large number of Congo red used in textiles, Congo red wastewater was not easily degraded, resulting in environmental and health-related problems. In order to improve the degradation efficiency of Congo red wastewater, A novel intimately coupled photocatalysis and biodegradation (ICPB) system was prepared by coupling Rhodopseudomonas palustris (R. Palustris), carbon nanotube - silver modified titanium dioxide photocatalytic composite (CNT-Ag -TiO2, CAT) and sodium alginate (SA) (R. palustris/CAT@SA). Compared with immobilized CAT and R. palustris, the R. palustris/CAT@SA improved the degradation and removal rates of Congo red by 14.3% and 42.1%, and the COD removal rates by 76% and 44.6%, respectively. The mechanism of the degradation of Congo red by the new ICPB was that the Congo red on the surface of the support was degraded into long-chain alkanes by the superoxide and hydroxyl radicals of CAT product, and then the long-chain alkanes were completely mineralization by R. Palustris, which reduced the accumulation of intermediates in the photocatalysis. Most of the Congo red was adsorbed to the interior of the carrier was degraded into aromatic hydrocarbons by R. Palustris, and then oxidized and degraded by CAT, and a small part of the Congo red would be directly mineralized by R. Palustris. A novel technical solution of R. palustris/CAT@SA provided a potential application to the degradation of dye wastewater.

Influence of biochar remediation on Eisenia fetida in Pb-contaminated soils.

In this study, the remediation influence of maize straw biochar on earthworms (Eisenia fetida) in contaminated soils (with Pb at 0, 300, 700, and 1000 mg kg-1) amended with different amounts of biochar (0%, 1%, 3%, and 5%) was investigated. The results showed that applying biochar to metal-polluted soils effectively reduced the mobility of Pb, promoting the transformation of Pb from exchangeable (EXC) and bound-to-carbonate (Carb) fractions to Fe/Mn oxide (FeMnOx), organic bound (ORG) and residual (RES) fractions. Consequently, a reduction in the mortality and weight loss of earthworms was also achieved by biochar. The accumulation amount of Pb in earthworms steadily increased with exposure time, and with the increasing dosage of biochar, the accumulated Pb decreased by 50.8-78.0% (300 mg kg-1), 30.9-67.3% (700 mg kg-1), and 17.4-55.1% (1000 mg kg-1), which was significantly positively correlated with the mortality of earthworms. Simultaneously, the application of biochar increased the soil pH (0.05-0.23 units), cation exchange capacity (CEC) (0.26-4.54 cmol kg-1), and content of organic matter (0.54-11.66%). There were higher soil enzyme activities (including sucrase activity, urease activity, and alkaline phosphatase activity) in the treatments with a biochar addition of 3%. Through remediation, Proteobacteria (50.82%), Actinobacteriota (32.37%), Firmicutes (4.83%) and Bacteroidota (1.88%) were the most important phyla in the microbiota communities. Furthermore, soil pH value and leaching toxicity concentration showed the most striking effects on earthworms. Therefore, the influence of earthworms must be taken into account in the remediation of Pb-contaminated soil with biochar.

Improving oxygen reduction reaction of microbial fuel cell by titanium dioxide attaching to dual metal organic frameworks as cathode.

In this study, a two-step simple distributed feeding method was used to prepare the core-shell nanocomposite dual metal organic frameworks (D-MOFs, TiO2@ZIF-67/ZIF-8). There were three obvious peaks (011), (112), (222) interface in D-MOFs core, which fully showed that ZIF-67/ZIF-8 crystal core was successfully synthesized. The morphology of composite material was core-shell structure with a rough surface, and Ti, Co, Zn, Al were uniformly distributed on the surface. TiO2@ZIF-67/ZIF-8 also had excellent electrochemical activity and the maximum power density of TiO2@ZIF-67/ZIF-8 microbial fuel cell (MFC) was 341.506 mW/m2, which was 1.30 times of ZIF-67/ZIF-8-MFC (262.144 mW/m2) and 2.07 times of ZIF-67-MFC (164.836 mW/m2). And the continuous output voltage of TiO2@ZIF-67/ZIF-8-MFC was 413.43 mV, which could maintain stable voltage output for 8.3 days. D-MOFs as the core of composites ensured the integrity, stability and high activity of materials; Rough TiO2 as the surface of the material provided surface area and reaction center.